The Tykes may hand a debut to winger Paddy McCourt, who joined the club last week.
Southampton could give a first start to record signing Daniel Osvaldo, who arrived from AS Roma this month.
Midfielder Morgan Schneiderlin is a fitness doubt and may not feature, while Luke Shaw has a knee problem which could prevent him from playing.
The outsider’s view of tonight’s contest at Oakwell would be that it offers a welcome distraction from the rigours of what has so far been a trying start to the Championship season.
The Reds have yet to register a win and have been on ther receiving end of two heavy defeats, most recently a 5-2 reverse at Blackburn.
Norway international Marcus Pedersen is expected to be handed his full debut after stepping off the substitutes' bench on Saturday, while fellow new signing Paddy McCourt is hoping to make his first appearance.
Midfielder Kelvin Etuhu made his first start at the weekend following a hamstring injury and should continue, but defender Martin Cranie (knee) remains sidelined.
Record signing Dani Osvaldo could make his first Southampton start.
Even in progressing to tonight’s second round of the Capital One Cup, where they meet in-form Southampton, Barnsley required a penalty shoot-out to see off League Two side Scunthorpe United.
But instead of sending his players out to enjoy themselves without the pressure of fighting for League points, Flitcroft wants his side to put into practice the positive work they have been doing on the training ground.
“We don’t need a distraction, we need a focus,” said the Reds chief.
“We need to focus on the training ground and we need to focus on our job. Distractions only bring negativity.”
Referee: S Duncan (Northumberland).
