Gordon Strachan's men can not qualify for next summer's tournament in Brazil, but will want to restore some pride ahead of qualifying for the European Championships in 2016.
The Scotland manager has already named his starting eleven, with James Morrison captaining the side and Leigh Griffiths earning his first start up front.
Croatia meanwhile, are level on points with Belgium at the top of the group, but will be without Real Madrid playmaker Luka Modric for tonight's game. Former Tottenham Hotspur defender Vedran Corluka is also missing.
Scotland boss Gordon Strachan has a host of key players missing for the clash as Darren Fletcher, Steven Fletcher, Kenny Miller, Scott Brown, James Forrest, Charlie Mulgrew, Danny Fox, Lee Wallace and Gary Caldwell are all ruled out.
West Brom midfielder James Morrison has been named as Scotland's captain for the tie.
"It's going to be hard. The squad is young. Some of our players don't even play European football, so it's imperative they get some kind of training for big games," Strachan said.
"But, for all our hard work, we know we are playing against world class players."
Croatia are missing two key players in Luka Modric and Vedran Corluka, who are both suspended.
Dinamo Zagreb's 16-year-old starlet Alen Halilovic, who has been linked with a number of top European clubs, is included in Croatia's squad.
